RTF2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1E8]

CAT#: TA502375AM

C20orf43 mouse monoclonal antibody, clone OTI1E8 (formerly 1E8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C20orf43 (RC201652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf43. Positive lysates LY414003 (100ug) and LC414003 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C20orf43 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C20orf43 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502375)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C20orf43 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502375)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-C20orf43 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502375)

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  Anti-C20orf43 mouse monoclonal antibody (TA502375) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C20orf43 (RC201652).

• HEK293T cells transfected with either RC201652 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C20orf43 antibody (TA502375), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1E8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C20orf43 (NP_057491) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: replication termination factor 2
• Database Link:
  NP_057491
  Entrez Gene 51507 Human
  UniProt ID Q9BY42
• Synonyms: C20orf43; CDAO5; HSPC164; SHUJUN-3