VCAM1 Mouse Monoclonal Antibody [Clone ID: OTI2G5]

CAT#: TA502411

VCAM1 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Positive lysates LY421491 (100ug) and LC421491 (20ug) can be purchased separately from OriGene.

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  Anti-VCAM1 mouse monoclonal antibody (TA502411) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1 (RC209761).

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  HEK293T cells transfected with either RC209761 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VCAM1 antibody (TA502411), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2G5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VCAM1 (NP_001069) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.7 kDa
• Gene Name: vascular cell adhesion molecule 1
• Database Link:
  NP_001069
  Entrez Gene 7412 Human
  UniProt ID P19320
• Background: This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: CD106; INCAM-100

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration