NNMT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A1]

CAT#: TA502470BM

NNMT mouse monoclonal antibody, clone OTI1A1 (formerly 1A1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT. Positive lysates LY401860 (100ug) and LC401860 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502470)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502470)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502470)

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  Anti-NNMT mouse monoclonal antibody (TA502470) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT (RC200641).

• HEK293T cells transfected with either RC200641 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NNMT antibody (TA502470), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NNMT (NP_006160) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: nicotinamide N-methyltransferase
• Database Link:
  NP_006160
  Entrez Gene 18113 MouseEntrez Gene 300691 RatEntrez Gene 489396 DogEntrez Gene 4837 Human
  UniProt ID P40261
• Background: N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]
• Synonyms: nicotinamide N-methyltransferase

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism