VASP Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C3]

CAT#: TA502492BM

VASP mouse monoclonal antibody, clone OTI1C3 (formerly 1C3), HRP conjugated

Product Images

HEK293T cells transfected with either RC203544 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VASP antibody (TA502492), and then analyzed by flow cytometry.

• 

  Anti-VASP mouse monoclonal antibody (TA502492) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP (RC203544).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (RC203544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP. Positive lysates LY401150 (100ug) and LC401150 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and VASP-Knockout HeLa cells (KO, Cat# LC832038) were separated by SDS-PAGE and immunoblotted with anti-VASP monoclonal antibody TA502492 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Show all 5 images

Specifications

Product Data

• Clone Name: OTI1C3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VASP (NP_003361) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.6 kDa
• Gene Name: vasodilator stimulated phosphoprotein
• Database Link:
  NP_003361
  Entrez Gene 22323 MouseEntrez Gene 361517 RatEntrez Gene 7408 Human
  UniProt ID P50552
• Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]
• Synonyms: vasodilator-stimulated phosphoprotein

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration