NNMT Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2G8]

CAT#: TA502522AM

NNMT mouse monoclonal antibody, clone OTI2G8 (formerly 2G8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT. Positive lysates LY401860 (100ug) and LC401860 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NNMT-Knockout HeLa cells (KO, Cat# LC832761) were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody TA502522 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502522)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502522)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502522)

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Specifications

Product Data

• Clone Name: OTI2G8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NNMT(NP_006160) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: nicotinamide N-methyltransferase
• Database Link:
  NP_006160
  Entrez Gene 18113 MouseEntrez Gene 300691 RatEntrez Gene 4837 Human
  UniProt ID P40261
• Background: N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]
• Synonyms: nicotinamide N-methyltransferase

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism