GBA3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C7]

CAT#: TA502604AM

GBA3 mouse monoclonal antibody, clone OTI1C7 (formerly 1C7), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3. Positive lysates LY402815 (100ug) and LC402815 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC211035 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody (TA502604), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-GBA3 antibody (TA502604), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-GBA3 antibody (TA502604), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Specifications

Product Data

• Clone Name: OTI1C7
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3 (NP_066024) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: glucosylceramidase beta 3 (gene/pseudogene)
• Database Link:
  NP_066024
  Entrez Gene 57733 Human
  UniProt ID Q9H227
• Background: GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]). [supplied by OMIM]
• Synonyms: CBG; CBGL1; GLUC; KLRP

Reference Data

• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism