Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: OTI1F1]

CAT#: TA502643

BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)

Product Images

Anti-BECN1 mouse monoclonal antibody (TA502643) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1 (RC201629).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BECN1 (Cat# RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1(Cat# TA502643). Positive lysates LY401239 (100ug) and LC401239 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.

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  HEK293T cells transfected with either RC201629 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody (TA502643), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-BECN1 antibody (TA502643), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Hela cells, using anti-BECN1 antibody (TA502643), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and BECN1-Knockout HeLa cells (KO, Cat# LC834634) were separated by SDS-PAGE and immunoblotted with anti-BECN1 monoclonal antibody TA502643 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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Specifications

Product Data

• Clone Name: OTI1F1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.35 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.7 kDa
• Gene Name: beclin 1
• Database Link:
  NP_003757
  Entrez Gene 56208 MouseEntrez Gene 114558 RatEntrez Gene 480513 DogEntrez Gene 8678 Human
  UniProt ID Q14457
• Background: Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]). [supplied by OMIM]
• Synonyms: ATG6; beclin1; VPS30

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy