PDLIM2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI10C4]

CAT#: TA502708AM

PDLIM2 mouse monoclonal antibody, clone OTI10C4 (formerly 10C4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDLIM2 (Cat# RC210022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM2(Cat# TA502708). Positive lysates LY411957 (100ug) and LC411957 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (30ug per lane) from 8 cell lines lysates by using anti-PDLIM2 monoclonal antibody (TA502708, 1:2000).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDLIM2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-PDLIM2 mouse monoclonal antibody (TA502708) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDLIM2 (RC210022).

Specifications

Product Data

• Clone Name: OTI10C4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDLIM2(NP_067643) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: PDZ and LIM domain 2
• Database Link:
  NP_067643
  Entrez Gene 486123 DogEntrez Gene 708652 MonkeyEntrez Gene 64236 Human
  UniProt ID Q96JY6
• Synonyms: MYSTIQUE; SLIM