PDLIM2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1D12]

CAT#: TA502709AM

PDLIM2 mouse monoclonal antibody, clone OTI1D12 (formerly 1D12), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM2 (RC210022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM2. Positive lysates LY411957 (100ug) and LC411957 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDLIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502709)

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  Anti-PDLIM2 mouse monoclonal antibody (TA502709) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDLIM2 (RC210022).

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  HEK293T cells transfected with either RC210022 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDLIM2 antibody (TA502709), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PDLIM2 antibody (TA502709), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PDLIM2 antibody (TA502709), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI1D12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDLIM2 (NP_067643) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: PDZ and LIM domain 2
• Database Link:
  NP_067643
  Entrez Gene 64236 Human
  UniProt ID Q96JY6
• Synonyms: MYSTIQUE; SLIM