HDHD2 Mouse Monoclonal Antibody [Clone ID: OTI1A5]

CAT#: TA502847

HDHD2 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD2 (RC205967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD2. Positive lysates LY403144 (100ug) and LC403144 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-HDHD2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDHD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502847)

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  HEK293T cells transfected with either RC205967 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDHD2 antibody (TA502847), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HDHD2(NP_115500) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.52 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.4 kDa
• Gene Name: haloacid dehalogenase like hydrolase domain containing 2
• Database Link:
  NP_115500
  Entrez Gene 84064 Human
  UniProt ID Q9H0R4
• Synonyms: 3110052N05Rik; HEL-S-301