MRAS Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3C4]

CAT#: TA502864AM

MRAS mouse monoclonal antibody, clone OTI3C4 (formerly 3C4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRAS (RC212259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRAS. Positive lysates LY415896 (100ug) and LC415896 (20ug) can be purchased separately from OriGene.

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  Anti-MRAS mouse monoclonal antibody (TA502864) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRAS (RC212259 ).

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  Flow cytometric Analysis of Jurkat cells, using anti-MRAS antibody (TA502864), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI3C4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MRAS (NP_036351) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.7 kDa
• Gene Name: muscle RAS oncogene homolog
• Database Link:
  NP_036351
  Entrez Gene 22808 Human
  UniProt ID O14807
• Background: Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. [supplied by OMIM]
• Synonyms: M-RAs; R-RAS3; RRAS3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction