Monoacylglycerol Lipase (MGLL) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A1]

CAT#: TA502887BM

MGLL (Monoacylglycerol Lipase) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGLL (RC218358, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGLL. Positive lysates LY402124 (100ug) and LC402124 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MGLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502887)

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  HEK293T cells transfected with either RC218358 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MGLL antibody (TA502887), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-MGLL antibody (TA502887), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI1A1
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MGLL (NP_009214) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.1 kDa
• Gene Name: monoglyceride lipase
• Database Link:
  NP_009214
  Entrez Gene 11343 Human
  UniProt ID Q99685
• Background: Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]). [supplied by OMIM]
• Synonyms: HU-K5; HUK5; MAGL; MGL

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways