COASY Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D5]

CAT#: TA503060AM

COASY mouse monoclonal antibody, clone OTI3D5 (formerly 3D5), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY. Positive lysates LY403068 (100ug) and LC403068 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Hela cells, using anti-COASY antibody (TA503060), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-COASY antibody (TA503060), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI3D5
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COASY (NP_079509) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.1 kDa
• Gene Name: Coenzyme A synthase
• Database Link:
  NP_079509
  Entrez Gene 71743 MouseEntrez Gene 287711 RatEntrez Gene 80347 Human
  UniProt ID Q13057
• Background: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM]
• Synonyms: DPCK; NBIA6; NBP; pOV-2; PPAT; UKR1

Reference Data

• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis