beta IV Tubulin (TUBB4A) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8E3]

CAT#: TA503110BM

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI8E3 (formerly 8E3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB4 (RC203945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB4. Positive lysates LY401833 (100ug) and LC401833 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB4 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

• Anti-TUBB4 mouse monoclonal antibody (TA503110) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB4 (RC203945).

• Flow cytometric Analysis of Hela cells, using anti-TUBB4 antibody (TA503110), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-TUBB4 antibody (TA503110), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI8E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100, IHC: 1:150
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBB4 (NP_006078) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: tubulin beta 4A class IVa
• Database Link:
  NP_006078
  Entrez Gene 22153 MouseEntrez Gene 29213 RatEntrez Gene 703019 MonkeyEntrez Gene 10382 Human
  UniProt ID P04350
• Synonyms: beta-5; DYT4; TUBB4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection