OSBP1 (OSBP) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F2]

CAT#: TA503140BM

OSBP mouse monoclonal antibody, clone OTI1F2 (formerly 1F2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP (RC204250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP. Positive lysates LY419260 (100ug) and LC419260 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503140)

• Anti-OSBP mouse monoclonal antibody (TA503140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP (RC204250).

• HEK293T cells transfected with either RC204250 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OSBP antibody (TA503140), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1F2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human OSBP (NP_002547) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89.2 kDa
• Gene Name: oxysterol binding protein
• Database Link:
  NP_002547
  Entrez Gene 76303 MouseEntrez Gene 365410 RatEntrez Gene 5007 Human
  UniProt ID P22059
• Background: Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq].
• Synonyms: OSBP1

Reference Data

• Protein Families: Druggable Genome