DPP3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2D9]

CAT#: TA503242AM

DPP3 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9), Biotinylated

Product Images

HEK293T cells transfected with either RC219658 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP3 antibody (TA503242), and then analyzed by flow cytometry.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP3 (RC219658, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP3. Positive lysates LY417120 (100ug) and LC417120 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPP3 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DPP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503242)

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Specifications

Product Data

• Clone Name: OTI2D9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPP3(NP_005691) produced in HEK293 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.4 kDa
• Gene Name: dipeptidyl peptidase 3
• Database Link:
  NP_005691
  Entrez Gene 75221 MouseEntrez Gene 10072 Human
  UniProt ID Q9NY33
• Background: This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]
• Synonyms: DPPIII

Reference Data

• Protein Families: Druggable Genome, Protease