SIRT5 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI 1G9]

CAT#: TA503279AM

SIRT5 mouse monoclonal antibody, clone OTI 1G9 (formerly 1G9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5. Positive lysates LY415885 (100ug) and LC415885 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SIRT5-Knockout HeLa cells (KO, Cat# LC830483) were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody TA503279 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  HEK293T cells transfected with either RC200189 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody (TA503279), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-SIRT5 antibody (TA503279), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-SIRT5 antibody (TA503279), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI 1G9
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIRT5 (NP_036373) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sirtuin 5
• Database Link:
  NP_036373
  Entrez Gene 68346 MouseEntrez Gene 306840 RatEntrez Gene 23408 Human
  UniProt ID Q9NXA8
• Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]
• Synonyms: SIR2L5

Reference Data

• Protein Families: Druggable Genome, Transcription Factors