Apg3 (ATG3) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3G3]

CAT#: TA503299BM

ATG3 mouse monoclonal antibody, clone OTI3G3 (formerly 3G3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3. Positive lysates LY411559 (100ug) and LC411559 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ATG3-Knockout 293T cells (KO, Cat# LC812022) were separated by SDS-PAGE and immunoblotted with anti-ATG3 monoclonal antibody TA503299, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Anti-ATG3 mouse monoclonal antibody (TA503299) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3 (RC203453).

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  HEK293T cells transfected with either RC203453 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATG3 antibody (TA503299), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3G3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: autophagy related 3
• Database Link:
  NP_071933
  Entrez Gene 67841 MouseEntrez Gene 171415 RatEntrez Gene 478564 DogEntrez Gene 708305 MonkeyEntrez Gene 64422 Human
  UniProt ID Q9NT62
• Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM, Mar 2008]
• Synonyms: APG3; APG3-LIKE; APG3L; PC3-96

Reference Data

• Protein Pathways: Regulation of autophagy