UBOX5 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C5]

CAT#: TA503340BM

UBOX5 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBOX5 (RC208505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBOX5.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UBOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503340)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-UBOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503340)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503340)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503340)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503340)

• Anti-UBOX5 mouse monoclonal antibody (TA503340) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBOX5 (RC208505).

• HEK293T cells transfected with either RC208505 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBOX5 antibody (TA503340), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-UBOX5 antibody (TA503340), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-UBOX5 antibody (TA503340), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1C5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, FLOW 1:100, IF: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-130 and C 419-487 of human UBOX5(NP_955447) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: U-box domain containing 5
• Database Link:
  NP_955447
  Entrez Gene 22888 Human
  UniProt ID O94941
• Synonyms: hUIP5; RNF37; UBCE7IP5; UIP5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis