SETD7 Mouse Monoclonal Antibody [Clone ID: OTI2E3]

CAT#: TA503377

SETD7 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (RC219244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7. Positive lysates LY403067 (100ug) and LC403067 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SETD7-Knockout HeLa cells (KO, Cat# LC833317) were separated by SDS-PAGE and immunoblotted with anti-SETD7 monoclonal antibody TA503377 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

• 

  HEK293T cells transfected with either RC219244 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SETD7 antibody (TA503377), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E3
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SETD7(NP_085151) produced in HEK293 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.31 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.5 kDa
• Gene Name: SET domain containing 7, histone lysine methyltransferase
• Database Link:
  NP_085151
  Entrez Gene 73251 MouseEntrez Gene 689954 RatEntrez Gene 80854 Human
  UniProt ID Q8WTS6
• Synonyms: 9; KMT7; SET7; SET9

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation