PGAM2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4E9]

CAT#: TA503425BM

PGAM2 mouse monoclonal antibody, clone OTI4E9 (formerly 4E9), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2. Positive lysates LY424823 (100ug) and LC424823 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503425)

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  Anti-PGAM2 mouse monoclonal antibody (TA503425) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2 (RC200701).

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  Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503425).

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  HEK293T cells transfected with either RC200701 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody (TA503425), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PGAM2(NP_000028) produced in HEK293 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.6 kDa
• Gene Name: phosphoglycerate mutase 2
• Database Link:
  NP_000281
  Entrez Gene 24959 RatEntrez Gene 56012 MouseEntrez Gene 720615 MonkeyEntrez Gene 5224 Human
  UniProt ID P15259
• Background: Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq]
• Synonyms: GSD10; PGAM-M; PGAMM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways