FDFT1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1H9]

CAT#: TA503450AM

FDFT1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FDFT1 (RC201392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FDFT1. Positive lysates LY401419 (100ug) and LC401419 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FDFT1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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  Western blot analysis of extracts (10ug) from a mouse tissues by using anti-FDFT1 monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and FDFT1-Knockout 293T cells (KO, Cat# LC841996) were separated by SDS-PAGE and immunoblotted with anti-FDFT1 monoclonal antibody TA503450, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503450)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503450)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503450)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503450)

• Anti-FDFT1 mouse monoclonal antibody (TA503450) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FDFT1 (RC201392).

• HEK293T cells transfected with either RC201392 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FDFT1 antibody (TA503450), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1H9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FDFT1(NP_004453) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.9 kDa
• Gene Name: farnesyl-diphosphate farnesyltransferase 1
• Database Link:
  NP_004453
  Entrez Gene 14137 MouseEntrez Gene 29580 RatEntrez Gene 2222 Human
  UniProt ID P37268
• Background: This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq]
• Synonyms: DGPT; ERG9; SQS; SS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Steroid biosynthesis