DTYMK Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1G8]

CAT#: TA503493AM

DTYMK mouse monoclonal antibody, clone OTI1G8 (formerly 1G8), Biotinylated

Product Images

HEK293T cells transfected with either RC201228 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DTYMK antibody (TA503493), and then analyzed by flow cytometry.

• 

  Anti-DTYMK mouse monoclonal antibody (TA503493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTYMK (RC201228).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTYMK (RC201228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTYMK. Positive lysates LY415933 (100ug) and LC415933 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DTYMK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503493)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DTYMK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503493)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DTYMK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503493)

• Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-DTYMK monoclonal antibody (1:200).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI1G8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DTYMK(NP_036277) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.6 kDa
• Gene Name: deoxythymidylate kinase
• Database Link:
  NP_036277
  Entrez Gene 1841 Human
  UniProt ID P23919
• Synonyms: CDC8; PP3731; TMPK; TYMK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism