EIF2B3 Mouse Monoclonal Antibody [Clone ID: OTI2H1]

CAT#: TA503537

EIF2B3 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2B3 (RC223570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2B3. Positive lysates LY412535 (100ug) and LC412535 (20ug) can be purchased separately from OriGene.

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  Anti-EIF2B3 mouse monoclonal antibody (TA503537) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF2B3 (RC223570).

Specifications

Product Data

• Clone Name: OTI2H1
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF2B3(NP_065098) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.31 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: eukaryotic translation initiation factor 2B subunit gamma
• Database Link:
  NP_065098
  Entrez Gene 108067 MouseEntrez Gene 171145 RatEntrez Gene 8891 Human
  UniProt ID Q9NR50
• Background: The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: EIF-2B; EIF2Bgamma