SEC14 like protein 2 (SEC14L2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F10]

CAT#: TA503577BM

SEC14L2 ( SEC14-like 2) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2. Positive lysates LY415756 (100ug) and LC415756 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503577)

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  Anti-SEC14L2 mouse monoclonal antibody (TA503577) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2 (RC215994).

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  HEK293T cells transfected with either RC215994 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody (TA503577), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1F10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SEC14L2(NP_036561) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: SEC14 like lipid binding 2
• Database Link:
  NP_036561
  Entrez Gene 67815 MouseEntrez Gene 23541 Human
  UniProt ID O76054
• Background: This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
• Synonyms: C22orf6; SPF; TAP; TAP1

Reference Data

• Protein Families: Transcription Factors