Heme oxygenase 2 (HMOX2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C2]

CAT#: TA503813AM

HMOX2 (Heme oxygenase 2) mouse monoclonal antibody, clone OTI1C2 (formerly 1C2), Biotinylated

Product Images

HEK293T cells transfected with either RC201777 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HMOX2 antibody (TA503813), and then analyzed by flow cytometry.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HMOX2 (Cat# RC201777, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMOX2(Cat# TA503813). Positive lysates LY419512 (100ug) and LC419512 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HMOX2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-HMOX2 monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI1C2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HMOX2(NP_002125) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.9 kDa
• Gene Name: heme oxygenase 2
• Database Link:
  NP_002125
  Entrez Gene 15369 MouseEntrez Gene 79239 RatEntrez Gene 3163 Human
  UniProt ID P30519
• Background: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq, Jul 2008]
• Synonyms: HO-2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Porphyrin and chlorophyll metabolism