NAPE PLD (NAPEPLD) Mouse Monoclonal Antibody [Clone ID: OTI4B12]

CAT#: TA503859

NAPEPLD (NAPE-PLD) mouse monoclonal antibody, clone OTI4B12 (formerly 4B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPEPLD (RC209877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPEPLD. Positive lysates LY404703 (100ug) and LC404703 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503859)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503859)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503859)

• 

  HEK293T cells transfected with either RC209877 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NAPEPLD antibody (TA503859), and then analyzed by flow cytometry.

Show all 5 images

Specifications

Product Data

• Clone Name: OTI4B12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human (NP_945341) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.74 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.4 kDa
• Gene Name: N-acyl phosphatidylethanolamine phospholipase D
• Database Link:
  NP_945341
  Entrez Gene 242864 MouseEntrez Gene 296757 RatEntrez Gene 222236 Human
  UniProt ID Q6IQ20
• Background: NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]). [supplied by OMIM]
• Synonyms: FMP30; NAPE-PLD