ERp72 (PDIA4) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2E2]

CAT#: TA503904BM

PDIA4 (ERp72) mouse monoclonal antibody, clone OTI2E2 (formerly 2E2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA4 (RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4. Positive lysates LY401531 (100ug) and LC401531 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-PDIA4 mouse monoclonal antibody (TA503904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDIA4 (RC204041).

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  HEK293T cells transfected with either RC204041 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDIA4 antibody (TA503904), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDIA4(NP_004902) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.8 kDa
• Gene Name: protein disulfide isomerase family A member 4
• Database Link:
  NP_004902
  Entrez Gene 12304 MouseEntrez Gene 116598 RatEntrez Gene 9601 Human
  UniProt ID P13667
• Synonyms: ERp-72; ERP70; ERP72

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection