PDSS2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2A3]

CAT#: TA503952BM

PDSS2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDSS2 (RC207892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDSS2. Positive lysates LY412512 (100ug) and LC412512 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDSS2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503952)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503952)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503952)

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  HEK293T cells transfected with either RC207892 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDSS2 antibody (TA503952), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2A3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDSS2(NP_065114) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: decaprenyl diphosphate synthase subunit 2
• Database Link:
  NP_065114
  Entrez Gene 71365 MouseEntrez Gene 365592 RatEntrez Gene 57107 Human
  UniProt ID Q86YH6
• Background: The protein encoded by this gene is an enzyme that synthesizes the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. The gene product catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. Defects in this gene are a cause of coenzyme Q10 deficiency. COMPLETENESS: complete on the 3' end.
• Synonyms: bA59I9.3; C6orf210; COQ10D3; DLP1; hDLP1

Reference Data

• Protein Pathways: Terpenoid backbone biosynthesis