APMAP Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3F2]

CAT#: TA504166BM

C20orf3 mouse monoclonal antibody, clone OTI3F2 (formerly 3F2), HRP conjugated

Product Images

Equivalent amounts of cell lysates (30 ug per lane) of wild-type HT-1080 cells (WT) and C20orf3-Knockdown HT-1080 cells (KD) were separated by SDS-PAGE and immunoblotted with anti-C20orf3 monoclonal antibody TA504166 (1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C20orf3 (RC202410, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf3. Positive lysates LY402795 (100ug) and LC402795 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C20orf3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-C20orf3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI3F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C20orf3(NP_065392) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.3 kDa
• Gene Name: adipocyte plasma membrane associated protein
• Database Link:
  NP_065392
  Entrez Gene 71881 MouseEntrez Gene 366227 RatEntrez Gene 57136 Human
  UniProt ID Q9HDC9
• Synonyms: BSCv; C20orf3

Reference Data

• Protein Families: Transmembrane