ALOX15 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2G3]

CAT#: TA504213BM

ALOX15 mouse monoclonal antibody, clone OTI2G3 (formerly 2G3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (RC206621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15. Positive lysates LY400456 (100ug) and LC400456 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ALOX15 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ALOX15 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  HEK293T cells transfected with either RC206621 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody (TA504213), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2G3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.6 kDa
• Gene Name: arachidonate 15-lipoxygenase
• Database Link:
  NP_001131
  Entrez Gene 246 Human
  UniProt ID P16050
• Synonyms: 12-LOX; 15-LOX-1; 15LOX-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways