ALG2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3C2]

CAT#: TA504242BM

ALG2 mouse monoclonal antibody, clone OTI3C2 (formerly 3C2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2. Positive lysates LY409737 (100ug) and LC409737 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALG2 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504242)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI3C2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALG2(NP_149078) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.9 kDa
• Gene Name: ALG2 alpha-1,3/1,6-mannosyltransferase
• Database Link:
  NP_149078
  Entrez Gene 85365 Human
  UniProt ID Q9H553
• Background: This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: CDGIi; hALPG2; NET38

Reference Data

• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis