ALOX15 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3G8]

CAT#: TA504250AM

ALOX15 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (RC206621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15. Positive lysates LY400456 (100ug) and LC400456 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALOX15 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Anti-ALOX15 mouse monoclonal antibody (TA504250) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15 (RC206621).

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Specifications

Product Data

• Clone Name: OTI3G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.6 kDa
• Gene Name: arachidonate 15-lipoxygenase
• Database Link:
  NP_001131
  Entrez Gene 81639 RatEntrez Gene 489458 DogEntrez Gene 709212 MonkeyEntrez Gene 246 Human
  UniProt ID P16050
• Synonyms: 12-LOX; 15-LOX-1; 15LOX-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways