DPP9 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1G9]

CAT#: TA504307AM

DPP9 mouse monoclonal antibody, clone OTI1G9 (formerly 1G9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP9 (RC224465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9. Positive lysates LY403380 (100ug) and LC403380 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPP9 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504307)

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  HEK293T cells transfected with either RC224465 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP9 antibody (TA504307), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-DPP9 antibody (TA504307), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1G9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPP9(NP_631898) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.4 kDa
• Gene Name: dipeptidyl peptidase 9
• Database Link:
  NP_631898
  Entrez Gene 224897 MouseEntrez Gene 485033 DogEntrez Gene 301130 RatEntrez Gene 695587 MonkeyEntrez Gene 91039 Human
  UniProt ID Q86TI2
• Background: This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq]
• Synonyms: DP9; DPLP9; DPRP-2; DPRP2

Reference Data

• Protein Families: Druggable Genome, Protease