Coatomer subunit delta (ARCN1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4H9]

CAT#: TA504314BM

ARCN1 mouse monoclonal antibody, clone OTI4H9 (formerly 4H9), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARCN1 (RC210778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARCN1. Positive lysates LY400624 (100ug) and LC400624 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARCN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504314)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504314)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504314)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504314)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504314)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504314)

• Anti-ARCN1 mouse monoclonal antibody (TA504314) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARCN1 (RC210778).

• HEK293T cells transfected with either RC210778 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARCN1 antibody (TA504314), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4H9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARCN1(NP_001646) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: archain 1
• Database Link:
  NP_001646
  Entrez Gene 213827 MouseEntrez Gene 300674 RatEntrez Gene 479415 DogEntrez Gene 372 Human
  UniProt ID P48444
• Background: This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq, Jul 2008]
• Synonyms: COPD