NLN Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3H4]

CAT#: TA504327AM

NLN mouse monoclonal antibody, clone OTI3H4 (formerly 3H4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (RC212447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN. Positive lysates LY412353 (100ug) and LC412353 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504327)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504327)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504327)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504327)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504327)

• HEK293T cells transfected with either RC212447 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NLN antibody (TA504327), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-NLN antibody (TA504327), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-NLN antibody (TA504327), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3H4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NLN(NP_065777) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.5 kDa
• Gene Name: neurolysin
• Database Link:
  NP_065777
  Entrez Gene 75805 MouseEntrez Gene 117041 RatEntrez Gene 478081 DogEntrez Gene 696884 MonkeyEntrez Gene 57486 Human
  UniProt ID Q9BYT8
• Background: This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract. [provided by RefSeq, Jun
• Synonyms: AGTBP; EP24.16; MEP; MOP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system