Cytochrome P450 2B6 (CYP2B6) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D5]

CAT#: TA504328AM

CYP2B6 (Cytochrome P450 2B6) mouse monoclonal antibody, clone OTI3D5 (formerly 3D5), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2B6 (RC217236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2B6. Positive lysates LY400259 (100ug) and LC400259 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYP2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Figure from citation: Western blot analysis of CYP2B6 protein level by using anti-CYP2B6 antibody in liver samples of patients with obstructive cholestasis. Dilution: 1:2000 View Citation

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504328)

• Anti-CYP2B6 mouse monoclonal antibody (TA504328) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2B6 (RC217236).

• HEK293T cells transfected with either RC217236 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2B6 antibody (TA504328), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-CYP2B6 antibody (TA504328), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3D5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CYP2B6(NP_000758) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.1 kDa
• Gene Name: cytochrome P450 family 2 subfamily B member 6
• Database Link:
  NP_000758
  Entrez Gene 474177 DogEntrez Gene 678687 MonkeyEntrez Gene 1555 Human
  UniProt ID P20813
• Background: This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
• Synonyms: CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6; EFVM; IIB1; P450

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism