Dystrobrevin beta (DTNB) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3H8]

CAT#: TA504332BM

DTNB (Dystrobrevin beta) mouse monoclonal antibody, clone OTI3H8 (formerly 3H8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTNB (RC203798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTNB. Positive lysates LY405251 (100ug) and LC405251 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DTNB monoclonal antibody.

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  Anti-DTNB mouse monoclonal antibody (TA504332) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DTNB (RC203798).

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  HEK293T cells transfected with either RC203798 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DTNB antibody (TA504332), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3H8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DTNB(NP_899205) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.6 kDa
• Gene Name: dystrobrevin beta
• Database Link:
  NP_899205
  Entrez Gene 13528 MouseEntrez Gene 362715 RatEntrez Gene 696167 MonkeyEntrez Gene 1838 Human
  UniProt ID O60941
• Background: This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: MGC17163; MGC57126