PSMD3 Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CAT#: TA504337

PSMD3 mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (RC202307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3. Positive lysates LY400996 (100ug) and LC400996 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC202307 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMD3 antibody (TA504337), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PSMD3 antibody (TA504337), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PSMD3 antibody (TA504337), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2A2
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMD3(NP_002800) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.95 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.8 kDa
• Gene Name: proteasome 26S subunit, non-ATPase 3
• Database Link:
  NP_002800
  Entrez Gene 22123 MouseEntrez Gene 287670 RatEntrez Gene 491018 DogEntrez Gene 698712 MonkeyEntrez Gene 5709 Human
  UniProt ID O43242
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq, Jul 2008]
• Synonyms: P58; RPN3; S3; TSTA2

Reference Data

• Protein Pathways: Proteasome