BCAT1 Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: TA504360S

BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5)

Product Images

Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and BCAT1-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-BCAT1 monoclonal antibody TA504360 (1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

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  Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human BCAT1 plasmid (RC219229, lane 2) using anti-BCAT1 antibody TA504360 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (50ug per lane) from 9 cell lines lysates by using anti-BCAT1 antibody. (TA504360, 1:500).

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  Western blot analysis of extracts (50ug per lane) from 10 cell lines lysates by using anti-BCAT1 monoclonal antibody(TA504360, 1:500)

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCAT1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BCAT1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCAT1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI3F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Dog, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.78 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.8 kDa
• Gene Name: branched chain amino acid transaminase 1
• Database Link:
  NP_005495
  Entrez Gene 12035 MouseEntrez Gene 486633 DogEntrez Gene 586 Human
  UniProt ID P54687
• Background: This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq]
• Synonyms: BCATC; BCT1; ECA39; MECA39; PNAS121; PP18

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation