RUVBL2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2B9]

CAT#: TA504384AM

RUVBL2 (Reptin/TIP49B) mouse monoclonal antibody, clone OTI2B9 (formerly 2B9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (RC200933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2. Positive lysates LY401993 (100ug) and LC401993 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RUVBL2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC200933 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody (TA504384), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2B9
• Applications: FC, WB
• Recommended Dilution: WB 1:1000~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: RuvB like AAA ATPase 2
• Database Link:
  NP_006657
  Entrez Gene 20174 MouseEntrez Gene 292907 RatEntrez Gene 476418 DogEntrez Gene 100428794 MonkeyEntrez Gene 10856 Human
  UniProt ID Q9Y230
• Background: This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq, Jul 2008]
• Synonyms: CGI-46; ECP51; INO80J; REPTIN; RVB2; TIH2; TIP48; TIP49B

Reference Data

• Protein Families: Transcription Factors