PSMB9 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F10]

CAT#: TA504389BM

PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB9 (RC209001, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB9. Positive lysates LY419098 (100ug) and LC419098 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC209001 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMB9 antibody (TA504389), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-PSMB9 antibody (TA504389), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI1F10
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9(NP_002791) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.1 kDa
• Gene Name: proteasome 20S subunit beta 9
• Database Link:
  NP_002791
  Entrez Gene 16912 MouseEntrez Gene 24967 RatEntrez Gene 5698 Human
  UniProt ID P28065
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar
• Synonyms: beta1i; LMP2; PSMB6i; RING12

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome