Carboxypeptidase A (CPA1) Mouse Monoclonal Antibody [Clone ID: OTI2E3]

CAT#: TA504517

CPA1 (Carboxypeptidase A1) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPA1 (RC202720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA1. Positive lysates LY419696 (100ug) and LC419696 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CPA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504517)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CPA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504517)

• 

  Anti-CPA1 mouse monoclonal antibody (TA504517) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CPA1 (RC202720).

• 

  HEK293T cells transfected with either RC202720 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CPA1 antibody (TA504517), and then analyzed by flow cytometry.

Show all 5 images

Specifications

Product Data

• Clone Name: OTI2E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CPA1(NP_001859) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.88 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.5 kDa
• Gene Name: carboxypeptidase A1
• Database Link:
  NP_001859
  Entrez Gene 1357 Human
  UniProt ID P15085
• Background: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a monomeric pancreatic exopeptidase involved in zymogen inhibition. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: CPA

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein