LIM1 (LHX1) Mouse Monoclonal Antibody [Clone ID: OTI2D5]

CAT#: TA504527

LHX1 (LIM1) mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX1 (RC210977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX1. Positive lysates LY417207 (100ug) and LC417207 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504527)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504527)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504527)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504527)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504527)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504527)

• Anti-LHX1 mouse monoclonal antibody (TA504527) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LHX1 (RC210977).

• HEK293T cells transfected with either RC210977 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LHX1 antibody (TA504527), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-LHX1 antibody (TA504527), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2D5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-362 of human LHX1(NP_005559) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.75 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.6 kDa
• Gene Name: LIM homeobox 1
• Database Link:
  NP_005559
  Entrez Gene 16869 MouseEntrez Gene 257634 RatEntrez Gene 3975 Human
  UniProt ID P48742
• Background: This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq]
• Synonyms: LIM-1; LIM1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors