LRRC50 (DNAAF1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2C4]

CAT#: TA504546AM

LRRC50 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50. Positive lysates LY405926 (100ug) and LC405926 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC205452 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LRRC50 antibody (TA504546), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2C4
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LRRC50(NP_848547) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 79.8 kDa
• Gene Name: dynein axonemal assembly factor 1
• Database Link:
  NP_848547
  Entrez Gene 68270 MouseEntrez Gene 361419 RatEntrez Gene 479628 DogEntrez Gene 715137 MonkeyEntrez Gene 123872 Human
  UniProt ID Q8NEP3
• Background: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. COMPLETENESS: complete on the 3' end.
• Synonyms: CILD13; LRRC50; ODA7; swt