C21ORF56 (SPATC1L) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1H6]

CAT#: TA504634AM

C21orf56 mouse monoclonal antibody, clone OTI1H6 (formerly 1H6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C21orf56 (RC224562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C21orf56. Positive lysates LY410249 (100ug) and LC410249 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-C21orf56 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C21orf56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504634)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C21orf56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504634)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C21orf56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504634)

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  HEK293T cells transfected with either RC224562 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C21orf56 antibody (TA504634), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-C21orf56 antibody (TA504634), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1H6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C21orf56(NP_115637) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.9 kDa
• Gene Name: spermatogenesis and centriole associated 1 like
• Database Link:
  NP_115637
  Entrez Gene 76573 MouseEntrez Gene 499418 RatEntrez Gene 607689 DogEntrez Gene 84221 Human
  UniProt ID Q9H0A9
• Synonyms: C21orf56