METT10D (METTL16) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3B5]

CAT#: TA504710BM

METT10D mouse monoclonal antibody, clone OTI3B5 (formerly 3B5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY METT10D (Cat# RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D(Cat# TA504710). Positive lysates LY411358 (100ug) and LC411358 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-METT10D monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-METT10D mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-METT10D mouse monoclonal antibody (TA504710) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY METT10D (RC208648).

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Specifications

Product Data

• Clone Name: OTI3B5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human METT10D(NP_076991) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.4 kDa
• Gene Name: methyltransferase 16, N6-methyladenosine
• Database Link:
  NP_076991
  Entrez Gene 67493 MouseEntrez Gene 721312 MonkeyEntrez Gene 79066 Human
  UniProt ID Q86W50
• Synonyms: METT10D