PADI4 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6A2]

CAT#: TA504887AM

PADI4 mouse monoclonal antibody, clone OTI6A2 (formerly 6A2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4. Positive lysates LY402202 (100ug) and LC402202 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504887)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504887)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504887)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504887)

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  Anti-PADI4 mouse monoclonal antibody (TA504887) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4 (RC206501).

• HEK293T cells transfected with either RC206501 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PADI4 antibody (TA504887), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6A2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amnio acids 299-588 of human PADI4(NP_036519) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.9 kDa
• Gene Name: peptidyl arginine deiminase 4
• Database Link:
  NP_036519
  Entrez Gene 23569 Human
  UniProt ID Q9UM07
• Background: This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq]
• Synonyms: PAD; PAD4; PADI5; PDI4; PDI5