COMMD1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3B3]

CAT#: TA504889AM

COMMD1 mouse monoclonal antibody,clone 3B3, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1 (RC205614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COMMD1. Positive lysates LY403473 (100ug) and LC403473 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-COMMD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-COMMD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COMMD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-COMMD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COMMD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• HEK293T cells transfected with either RC205614 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COMMD1 antibody (TA504889), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3B3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COMMD1(NP_689729) produced in HEK293T cell.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21 kDa
• Gene Name: copper metabolism domain containing 1
• Database Link:
  NP_689729
  Entrez Gene 17846 MouseEntrez Gene 289831 RatEntrez Gene 150684 Human
  UniProt ID Q8N668
• Background: COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]). [supplied by OMIM]
• Synonyms: C2orf5; MURR1