FTCD Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI7C5]

CAT#: TA504943AM

FTCD mouse monoclonal antibody,clone 7C5, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FTCD (RC208573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FTCD. Positive lysates LY416500 (100ug) and LC416500 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FTCD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC208573 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FTCD antibody (TA504943), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7C5
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FTCD(NP_006648) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.7 kDa
• Gene Name: formimidoyltransferase cyclodeaminase
• Database Link:
  NP_006648
  Entrez Gene 721818 MonkeyEntrez Gene 10841 Human
  UniProt ID O95954
• Background: The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2009]
• Synonyms: LCHC1

Reference Data

• Protein Pathways: Histidine metabolism, Metabolic pathways, One carbon pool by folate